The KESM 2 user guide (link below) provides detailed operational procedures and best practices when operating the instrument.

Daniel Miller, Jaewook Yoo and Yoonsuck Choe. Guide to using the Knife Edge Scanning Microscope (KESM 2.0). Technical Report 2017-8-1, Department of Computer Science & Engineering, Texas A&M University, 2017.

Also see the following publication for specimen preparation and further instructions.

Choe, Y., Mayerich, D., Kwon, J., Miller, D. E., Sung, C., Chung, J. R., Huffman, T., Keyser, J., and Abbott, L. C. (2011b). Specimen preparation, imaging, and analysis protocols for knife-edge scanning microscopy. Journal of Visualized Experiments, 58:e3248. Doi: 10.3791/3248.